Microbial Profiles in Saliva from Children with and without Caries in Mixed Dentition

Aihua Luo2, Bingchang Xin2, Bruce J. Paster3,4, Jie Qin1,2, Deqin Yang1,2,*DOI: 10.1111/j.1601-0825.2012.01915.x

© 2012 John Wiley & Sons A/S

Issue

Oral DiseasesAccepted Article (Accepted, unedited articles published online for future issues)

Additional Information

Author Information1

The Affiliated Stomatological Hospital of Chongqing Medical University, P.R.China, 401147

2

The Affiliated Stomatological Hospital of Zunyi Medical College, P.R.China, 563003

3

The Forsyth Institute, Cambridge, MA 02142, USA

4

The Harvard School of Dental Medicine, Boston MA 02115, USA

* Corresponding author:yangdeqin@gmail.com

Publication HistoryAccepted manuscript online: 11 FEB 2012 10:05AM ESTReceived Date : 03‐Nov‐2011 , Revised Date : 08‐Jan‐2012 , Accepted Date : 23‐Jan‐2012 SEARCH Search Scope All contentPublication titlesIn this journalIn this issue Search String Advanced >Saved Searches > SEARCH BY CITATION Volume: Issue: Page: ARTICLE TOOLSGet PDF (350K)Save to My ProfileE-mail Link to this ArticleExport Citation for this ArticleGet Citation AlertsRequest Permissions AbstractCited By Get PDF (350K) Keywords:dental caries;saliva;HOMIM;bacteria;isolated population

OBJECTIVE:  The purpose of the present study was to determine the bacterial profiles in saliva of the isolated children for studying caries etiology.

MATERIALS AND METHODS:  Samples were collected from isolated children from 6 to 8 years old including 20 caries‐free (dmfs=0) (Health) and 30 caries‐active individuals (dmfs>8) (Patients). 16S rRNA genes were amplified by PCR from bacterial DNA of saliva sample and labeled via incorporation of Cy3‐dCTP in second nested PCR. After hybridization of labeled amplicons on HOMIM, the microarray slides were scanned and original data acquired from professional software.

RESULTS:  Collectively, 94 bacterial species or clusters representing 6 bacterial phyla and 30 genera were detected. A higher bacterial diversity was observed in patients than in health. Statistical analyses revealed 8 species or clusters were detected more frequently in diseased patients than in healthy samples, while 6 different species were detected more frequently in healthy as compared to diseased patients.

CONCLUSION:  The diversity of microbe within saliva derived from isolated population increased in caries‐active status, there are some bacteria in salivary flora can be as candidate biomarkers for caries prognosis in mixed dentition. The imbalances in the resident microflora may be the ultimate mechanism of dental caries.

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